1 *, 1, 1, Tatiana A. Korshunova 2, 1, Alena B. Zuzina 1, Victor N. Ierusalimsky 1, 1, 2, Ivan A.

Bricscad license key serial number. • Immediate 3D modeling tasks support.

As part of this project, team members conduct IAQ trainings, host educational panel and conferences, created ecological clubs (EKOLOG), maintain a Facebook page, and have recorded plays available on You Tube. During the first year of this project Dr. Lipsett and her team were able to educate more than 6,000 students, teachers and parents.

Novikov 3, 1, 1, 1, 4, 4, Yekaterina Popova 5, 5 and 1 • 1Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow, Russia • 2Koltzov Institute of Developmental Biology, Moscow, Russia • 3Research Institute of Eye Diseases, Moscow, Russia • 4Vavilov Institute of General Genetics, Russia Academy of Sciences, Moscow, Russia • 5Space Biosciences Research of NASA Ames Research Center, Moffett Field, CA, United States The vestibular system receives a permanent influence from gravity and reflexively controls equilibrium. If we assume gravity has remained constant during the species' evolution, will its sensory system adapt to abrupt loss of that force? We address this question in the land snail Helix lucorum exposed to 30 days of near weightlessness aboard the Bion-M1 satellite, and studied geotactic behavior of postflight snails, differential gene expressions in statocyst transcriptome, and electrophysiological responses of mechanoreceptors to applied tilts.

Each approach revealed plastic changes in the snail's vestibular system assumed in response to spaceflight. Absence of light during the mission also affected statocyst physiology, as revealed by comparison to dark-conditioned control groups. Readaptation to normal tilt responses occurred at ~20 h following return to Earth. Despite the permanence of gravity, the snail responded in a compensatory manner to its loss and readapted once gravity was restored. Behavioral Studies Gravitaxis reaction to a 90° head down pitch from horizontal was tested in a previously described apparatus () following the same protocol, and full course of this stereotypical behavior was recorded for later analysis using a general purpose digital camera at 30 fps. To provide a double blind control analysis the filenames of the PF, ST, and NV groups were encrypted.

Three of the authors (M. Z.) independently split video timelines for each snail to fit the gravitaxis phases as described in the previous report (). The three analyzers then met, reevaluated their tables of latencies, and made screenshots for every phase of each snail's response. A difference in response latencies of >5 s between the 3 datasets was found in about 3% of measures, thereby triggering a discussion of the merits of each measure until the best measure was determined. The other 2 experimental groups, DK and OL, were investigated roughly a year later and a double blind procedure was deemed not pertinent in this case.

Tentacle withdrawal reaction was measured as the length of each tentacle, first in pixels using ImageJ and then calculated and analyzed as a percentage of full tentacle length (corresponding to T0 phase of gravitaxis response). The angle in degrees between tentacles was measured in ImageJ for every phase of gravitaxis. Accuracy of ImageJ measurements from screenshots was evaluated by mean difference between results of 3 researchers (N. P.), and the data for 3 randomly chosen animals from PF group and 3 from any of control groups were used. For tentacle length it was 6% error of absolute values, and 4% for inter-tentacle angle measurement. All time parameters were measured with accuracy of 1 s by record timeline on screenshots.

SEM of Inertial Mass Scanning Electron Microscopy (SEM) analysis was made on statocysts from three groups of snails. The statocysts were extracted after the experimental session, frozen dry and stored at −80°C. Before SEM imaging statocysts were fixed by cold absolute ethanol for 12 h, rinsed in distilled water, and placed onto carbon tape in a drop of water. The statocyst was dissected under a dissecting microscope to visualize the inertial mass using fine neurosurgery forceps. After removal of overlying structures, the statoconia preparations were left for few minutes to desiccate. The prepared samples were then placed on carbon tape for SEM and put horizontally on the stage of the microscope (EVO LS10, Carl Zeiss, Germany) for backscattered electrons (BSE) imaging under low vacuum (50–70 Pa) and accelerating voltage of 22 kV (LaB6 cathode). Digital images were captured in tiff format with resolution 3,024 × 2,406 pixels.

Layout table for study information Study Type: Interventional (Clinical Trial) Actual Enrollment: 162 participants Allocation: Randomized Intervention Model: Parallel Assignment Masking: Double (Participant, Investigator) Primary Purpose: Treatment Official Title: Clinical Evaluation of Condrosulf 800 mg in the Treatment of Symptomatic OA of the Hand: a 6-month, Double-blind, Placebo Controlled Study Study Start Date: June 2005 Actual Primary Completion Date: December 2009 Actual Study Completion Date: August 2010. Condrosulf 800 otzivi. Secondary endpoints are: Efficacy evaluation: Global impression of efficacy expressed by the patient and the physician (VAS), Grip strength (measured manometrically); Morning stiffness duration; Consumption of Paracetamol; Tolerability (4-point verbal scale); Adverse Events occurring during the treatment period; Other parameters:Treatment compliance; Biological markers of arthritis. Inclusion Criteria: • Patients of either sex • Aged 40 and over • Outpatients • Patients fulfilling the ACR criteria for the reporting of hand OA • Patients showing X-ray features of hand OA on at least two joints (2 IP and/or 1 IP and 1 TMC) of the dominant hand on standard plain radiographs ( or = 40 mm at inclusion time). • Showing a FIHOA score > or = 6. Primary endpoints: Primary endpoints of the study are the evaluation of global spontaneous pain (Huskisson's Visual Analogue Scale: VAS) and the score of Dreiser's algo-functional index (FIHOA) during the treatment with the tested product, Condrosulf®, versus placebo.